Chemistry Practicals Class 12
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- In this paper chromatography experiment, you will understand chromatography, adsorption chromatography, partition chromatography, column chromatography, TLC, paper chromatography, and the retardation factor.
- You will understand the principle of adsorption chromatography and partition chromatography.
- Once you understand the concept of the experiment and the different steps involved, you can perform the experiment in the real lab more accurately and quickly.
- Using this 3D simulation, you can separate the mixture of inks using the suitable mobile phase.
- Using our chemistry virtual lab, you will understand the different components present in the extract of spinach leaves.
- You will be able to identify the components of the mixture based on their Rf value.
Paper chromatography is a technique used for the separation of dissolved chemical substances by taking advantage of their different rates of migration on the paper sheet. It is an inexpensive and powerful analytical method that requires a very small amount of substance for the separation. Paper chromatography works on the principle of partition. The paper acts as a stationary phase, and the solvent in which the paper is immersed acts as a mobile phase that separates the components of the sample.
In this method, the sample or substance is applied as a fine spot at the bottom of the paper. This paper is immersed in the solvent in which the components of the mixture are soluble in varying degrees. The solvent penetrates over the paper via capillary action. It passes over the sample spot and carries over the soluble components along with it. The substance moves with the solvent at different velocities depending on its solubility in that solvent.
The locations of separated components are identified by applying the reagents that form the coloured compounds with the separated substances. For example, in the separation of a mixture of amino acids, a Ninhydrin reagent is applied. Amino acids show different colours with a Ninhydrin reagent.
Watch this video to learn more about chemistry.
Requirements for this Science Experiment
⦁ Chromatography Chamber ⦁ Fine Capillary Tubes ⦁ Whatman Filter Paper Strip ⦁ Pencil ⦁ Scale ⦁ Measuring Cylinders ⦁ Beakers ⦁ Glass Rod