Chemistry Practicals Class 9

Separation of Dyes in Black Ink Using Chromatography

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About Simulation

  • Engage in an interactive and educational experience with this simulation of the paper chromatography experiment, where you will explore the virtual separation of colours within black ink.
  • By utilizing this simulation, you can acquire a hands-on understanding of the principles underlying paper chromatography. Experiment with various solvents and witness the dynamic separation of colours in black ink.
  • Learn the step-by-step process of preparing chromatography paper strips and applying samples of black ink, gaining practical insight into experimental procedures.
  • Delve into the factors that influence the movement of dyes during chromatography, such as the composition of the solvent and the properties of the chromatography paper.

Chemistry Practical Class

  • Through exploration, you can deepen your understanding of the intricate dynamics governing the separation process.

Simulation Details

Duration – 30 Minutes
Easily Accessible
Languages – Odia & English
Platforms – Android & Windows


Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. It can separate dissolved solids that are present in a solution in very small quantities.

Chromatography operates on the principle of separating molecules within a mixture by their differential interactions with a stationary phase and a mobile phase. Molecules are applied onto a surface or into a solid, and as the mobile phase moves, the components of the mixture separate from each other based on various factors. These factors include molecular characteristics such as adsorption (liquid-solid), partition (liquid-solid), and differences in affinity or molecular weights.

Based on this approach, three components form the basis of the chromatography technique.

  • Stationary phase: This phase is always composed of a “solid” phase or “a layer of a liquid adsorbed on the surface a solid support”.
  • Mobile phase: This phase is always composed of “liquid” or a “gaseous component.”
  • Separated molecules

The dye in the black ink is not a single colour, but it is a mixture of 2 or 3 colours. In other words, black ink is a mixture of different colour dyes (solute) in water (solvent), which can be easily identified with the help of paper chromatography.

Chemistry Practical Class

Paper chromatography is the technique used for the separation of those solutes that dissolve in the same solvent. The separation by this technique is based upon the principle that though two (or more) solutes are soluble in the same solvent, their solubilities may be different.

Watch this video to learn more about chemistry.

Requirements for this Science Experiment

Black ink pen Distilled water Chromatographic chamber Whatman filter paper strip Pencil Scale

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