Biology Practicals Class 9

Preparation of Stained Onion Peel Slide

  • Teach science experiments in a gamified way
  • Boost conceptual clarity and knowledge retention
  • Aligned with National Education Policy 2020
  • Helpful in getting NAAC accreditation
  • CBSE, ICSE, and state boards aligned curricula
  • Engaging simulations with easy-to-teach instructions

About Simulation

  • The experiment focusing on the onion peel provides a hands-on opportunity for learners to explore and understand the unique characteristics of plant cells, such as their presence of a large central vacuole, rectangular shape, and cell wall, which distinguish them from animal cells.
  • Through engaging and simplified experiments, participants can experience the dynamics of biology firsthand, gaining insights into the structure and function of plant cells and the role of organelles within them.
  • By studying the microscopic structure of the onion peel in the simulation, learners can develop a deeper appreciation for the complexity and diversity of plant biology, enhancing their understanding of cellular processes and structures.

Biology Practical Class

  • The simulation also covers the staining procedure for onion slide preparation, offering learners a comprehensive understanding of the techniques used in microscopy to visualize and study cellular components effectively. This knowledge can be valuable for future experiments and research in the field of biology.

Simulation Details

Duration – 30 Minutes
Easily Accessible
Languages – Odia & English
Platforms – Android & Windows


An onion peel is a complex multicellular organism characterized by a cell wall, membrane, cytoplasm, nucleus, and a large vacuole. The cell wall is made of cellulose, a tough and rigid polysaccharide that provides structural support and protection against external stress. The membrane, or plasma membrane, is a thin and flexible layer of lipids and proteins that surrounds the cell, regulating the movement of molecules in and out of the cell through selective permeability. The cytoplasm is the gel-like substance that fills the cell, containing various organelles such as mitochondria, ribosomes, and the endoplasmic reticulum that carry out specific functions such as energy production, protein synthesis and lipid metabolism.

The cell nucleus controls the growth, development, and reproduction of the cell. The membrane-bound vacuole stores water, nutrients, and waste products, and maintains the cell’s shape and structure. Cheek cells lack a wall and have a semi-permeable membrane that allows nutrient and waste exchange. They have a denser cytoplasm due to the presence of many mitochondria and a smaller vacuole compared to onion cells. Understanding each cell type’s unique features is crucial for understanding their functions and adaptations. The process of obtaining an onion peel for microscopic examination involves carefully separating the thin, transparent layers of the onion bulb to reveal the cells within. Onion peel technique allows for detailed observation of the cell structure and organization.

Requirements for this Science Experiment

  • A medium-sized onion
  • A watch glass
  • Two slides
  • Coverslip
  • Forceps
  • Sharp knife
  • Needle
  • Dropper
  • Blotting paper
  • Glycerine
  • Distilled water
  • Safranin
  • Compound microscope

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